Unbiased protein association study on the public human proteome reveals biological connections between co-occurring protein pairs

Mass-spectrometry-based, high-throughput proteomics experiments produce large amounts of data. While typically acquired to answer specific biological questions, these data can also be reused in orthogonal wayS to reveal new biological knowledge. We here present a novel method for such orthogonal data reuse of public proteomics data. Our method elucidates biological relationships between proteins based on the co-occurrence of these proteins across human experiments in the PRIDE database. The majority of the significantly co-occurring protein pairs that were detected by our method have been successfully mapped to existing biological knowledge. The validity of our novel method is substantiated by the extremely few pairs that can be mapped to existing knowledge based on random associations between the same set of proteins. Moreover, using literature searches and the STRING database, we were able to derive meaningful biological associations for unannotated protein pairs that were detected using our method, further illustrating that as-yet unknown associations present highly interesting targets for follow-up analysis

Bènard-Marangoni Convection with Free Slip Bottom and Mixed Thermal Boundary Conditions

The onset of cellular convection induced by surface tension gradients in a horizontal liquid layer heated from below is examined by making use of linear stability analysis for mixed thermal boundary conditions with the free-slip condition at the lower boundary. We use a combination of analytical and numerical techniques to obtain a detailed description of marginal stability curves. It is established numerically that ‘the principle of exchange of stabilities’ is valid. The numerical results are presented for a wide range of the values of the parameters characterizing the nature of thermal boundary conditions. We investigate for the first time, a situation wherein value of the parameter characterizing the thermal condition at the upper boundary varies inversely to that characterizing the thermal condition at the lower boundary, and obtained distinct ranges in which increasing values of the parameter of the lower boundary lead to formation of convection cells of increasing or decreasing size at the onset of convection

The online Tabloid Proteome : an annotated database of protein associations

A complete knowledge of the proteome can only be attained by determining the associations between proteins, along with the nature of these associations (e.g. physical contact in protein–protein interactions, participation in complex formation or different roles in the same pathway). Despite extensive efforts in elucidating direct protein interactions, our knowledge on the complete spectrum of protein associations remains limited. We therefore developed a new approach that detects protein associations from identifications obtained after re-processing of large-scale, public mass spectrometry-based proteomics data. Our approach infers protein association based on the co-occurrence of proteins across many different proteomics experiments, and provides information that is almost completely complementary to traditional direct protein interaction studies. We here present a web interface to query and explore the associations derived from this method, called the online Tabloid Proteome. The online Tabloid Proteome also integrates biological knowledge from several existing resources to annotate our derived protein associations. The online Tabloid Proteome is freely available through a user-friendly web interface, which provides intuitive navigation and data exploration options for the user at http://iomics.ugent.be/tabloidproteome

Comparative evaluation of FESS and septoplasty with FESS in cases of DNS with chronic maxillary sinusitis

Background: Present study compares basic FESS and septoplasty with FESS alone in DNS with maxillary sinusitis.Methods: Sixty patients of DNS with chronic maxillary sinusitis were divided into two groups alternatively. After pre-operative symptoms score and computerized tomography (CT scan), twenty patients underwent FESS with septoplasty (group A) and other 20 underwent FESS alone (group B) under local anaesthesia and topical 4% lignocaine with 1:1000 adrenaline. At 6 weeks, post-operative symptom score and CT scan findings were documented and compared statistically by using unpaired student t-test.Results: Ninety six percent of patients in group A and 87.6% in group B have shown complete improvement in facial pain/pressure. Ninety three percent of patients in group A and 83.3% in group B have shown complete improvement in headache. Ninety percent patients in group A and 63.3% in group B has shown complete improvement in nasal obstruction. Seventy six percent of patients in group A and 63.3% of patients in group B have shown complete improvement in nasal discharge. Eighty six percent and 63.3% of patients in group A and group B respectively were satisfied from the surgery. Ninety three percent of patients in group A and 70% in group B were found to have normal maxillary sinus mucosa on HRCT nose and PNS after 6 weeks following surgical treatment. Hundred percent patients in group A and 96.7% of patients in group B were found to have normal OMC on HRCT nose and PNS 6 weeks after surgery.Conclusions: It was observed that FESS with septoplasty is effective for the treatment of chronic rhinosinusitis with deviated nasal septum on VAS as well as radiologically (the Lund and Mackay staging system: radiologic staging) than FESS alone

Solubility Enhancement of Water Insoluble Drug for Ophthalmic Formulation

Product development of ophthalmic preparations has received considerable attention in the last few years. With the increasing emphasis on their sterility, some of the differences between them and parenteral preparations are becoming less evident. Both classes of preparations employ similar added substances and manufacturing procedures. The successful formulation of poorly water-soluble drugs is one of the major problems in pharmaceutical manufacturing. Poorly water-soluble drugs, such as indomethacin, may show low and erratic oral bioavailability due to poor dissolution of the drug in the fluids of the gastrointestinal tract. Indomethacin is a water insoluble drug, so problems of formulating an aqueous eye-drop are well known. Moreover, unstability of Indomethacin aqueous preparations is also a great challenge. In this research work, considering pharmacological importance of drug Indomethacin, we tried to overcome the problem of poor water solubility by making a salt of it and thus formulating an aqueous ophthalmic preparation.Keywords: ophthalmic preparation, indomethacin salt, aqueous eye drops, poor water soluble drugs

MAPPI-DAT : data management and analysis for protein-protein interaction data from the high-throughput MAPPIT cell microarray platform

Protein-protein interaction (PPI) studies have dramatically expanded our knowledge about cellular behaviour and development in different conditions. A multitude of high-throughput PPI techniques have been developed to achieve proteome-scale coverage for PPI studies, including the microarray based Mammalian Protein-Protein Interaction Trap (MAPPIT) system. Because such high-throughput techniques typically report thousands of interactions, managing and analysing the large amounts of acquired data is a challenge. We have therefore built the MAPPIT cell microArray Protein Protein Interaction-Data management & Analysis Tool (MAPPI-DAT) as an automated data management and analysis tool for MAPPIT cell microarray experiments. MAPPI-DAT stores the experimental data and metadata in a systematic and structured way, automates data analysis and interpretation, and enables the meta-analysis of MAPPIT cell microarray data across all stored experiments